The inactivation of rat adipocyte Mg2+-dependent phosphatidate phosphohydrolase by noradrenaline

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Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects.

A new procedure for the partial purification of Mg2+-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg2+-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl2, gel filtration on Sephacryl S-400, chromatography on DEAE-cellulose and affinity chromatography on calmodulin-agarose. From the parallel change in staining intensity and in th...

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The effects of steroid hormones, glucagon and insulin on rat liver phosphatidate phosphohydrolase (PAP) activity were studied both in vitro and in vivo. Incubation of rat hepatocytes with each hormone showed that dehydroepiandrosterone (DHEA), progesterone and testosterone increase PAP activity by 44.6, 37 and 36.9%, respectively. Estradiol, however, decreased enzyme activity by 13.6% under...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1980

ISSN: 0264-6021

DOI: 10.1042/bj1900659